Plant chromosomal high mobility group (HMG) proteins
نویسندگان
چکیده
منابع مشابه
High-mobility group chromosomal proteins of wheat.
Four proteins have been extracted from purified chromatin of wheat embryos with 0.35 M NaCl. These proteins are soluble in 2% (w/v) trichloroacetic acid and thus meet the original operational requirements to be classified as "high-mobility group" (HMG) chromosomal proteins. The proteins have been characterized by one- and two-dimensional electrophoresis, amino acid analysis, and peptide mapping...
متن کاملSEPARATION OF NONHISTONE HIGH MOBILITY GROUP (HMG) FROM HUMAN LYMPHOCYTES BY HIGH PERFORMANCE LIQUID CHROMATOGRAPHY
The high mobility group (HMG) of nonhistone proteins have been investigated using two high performance liquid chromatographic techniques (HPLC). Reversed-phase HPLC under conditions of 50 mM triethylamine adjusted to pH 2.2 with phosphoric acid (solvent A) and 95% acetonitrile in water (solvent B) was used to separate proteins primarily on the basis of differences in the overall hydrophobi...
متن کاملHeterogeneous binding of high mobility group chromosomal proteins to nuclei
A dramatic difference is observed in the intracellular distribution of the high mobility group (HMG) proteins when chicken embryo fibroblasts are fractionated into nucleus and cytoplasm by either mass enucleation of cytochalasin-B-treated cells or by differential centrifugation of mechanically disrupted cells. Nuclei (karyoplasts) obtained by cytochalasin B treatment of cells contain more than ...
متن کاملSubnucleosome particles containing high mobility group proteins HMG-E and HMG-G originate from transcriptionally active chromatin.
Subnucleosome particles SN2 and SN3 containing short DNA fragments and non-histone proteins of the high mobility group, HMG-G and HMG-E respectively, were purified from the chromatin preparations of mouse L cells partially digested with staphylococcal nuclease. Labeled DNAs prepared from these particles were hybridized to an excess of nuclear RNA. The binding of subnucleosomal DNA was about 3-f...
متن کاملUpdate on Plant HMG-Box Proteins Plant Proteins Containing High Mobility Group Box DNA-Binding Domains Modulate Different Nuclear Processes1[W]
The HMG-box is an approximately 75-amino acid residue protein domain that occurs in all eukaryotic organisms and was first identified as a characteristic feature of the chromosomal high mobility group (HMG) proteins of the HMGB type. Structural studies have demonstrated that the L-shaped fold of the domain formed by three a-helices is conserved to a greater extent than expected from amino acid ...
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ژورنال
عنوان ژورنال: The Plant Journal
سال: 1995
ISSN: 0960-7412,1365-313X
DOI: 10.1046/j.1365-313x.1995.7020185.x